The PrnaI sequences of plasmids are listed, with the −35 and −10 consensus sequences for canonical promoters boxed and base changes from the wild-type sequence shown in boldface. The acylation of BlaR1 sensor domain by the antibiotic generates an intramolecular signal that leads to the activation of the L3 cytoplasmic loop of the transmitter by a single-point cleavage. Replication frequency (and hence plasmid copy number) is therefore determined by the availability of active Rep molecules. The Pots & Pans cycle performed even better with those same stains, but couldn’t quite cut it against our burnt cheese test. Cell 59:395-404. CrossRefMedlineGoogle Scholar ↵ Paulsen, I. T., N. Firth, and R. A. Skurray. 1997. Resistance to antimicrobial agents other than β-lactams, p. 175-212. In K. B. Crossley and G. L. Archer (ed.), The staphylococci in human disease. View larger version: FIG. 3. Complex formation between RNAI antisense and rep mRNA leader transcripts.
Mol Microbiol 24: 1025–1037.PD GregoryRA LewisSP CurnockKG Dyke1997Studies of the repressor (BlaI) of beta-lactamase synthesis in Staphylococcus aureus.Mol Microbiol2410251037 23. Rawlings ND, Barrett AJ (1995) Evolutionary families of metallopeptidases. The mutation of this glutamate residue to aspartate (E213D) or to glutamine (E213Q) prevents β-lactamase induction (Table 1, Figure 3). These results demonstrate that all the residues of this conserved motif play an essential role in the induction process. The probe (88 bp) was generated by PCR using primers PEX4 and SK41-R19. Three independent DNA and RNA isolations are shown for each plasmid.
Click the target next to the incorrect Subject Area and let us know. All routine DNA isolation and manipulation were performed as described by Sambrook et al.  or following instructions of the manufacturer. Staphylococcus aureus multiresistance plasmid pSK41: analysis of the replication region, initiator protein binding and antisense RNA regulation — Kwong — 2004 — Molecular Microbiology — Wiley Online Library. American Society for Microbiology, Washington, D.C. Google Scholar ↵ Franch, T., M. Petersen, E. G. H. Wagner, J. P. Jacobsen, and K. Gerdes. 1999. Antisense RNA regulation in prokaryotes: rapid RNA/RNA interaction facilitated by a general U-turn loop structure. J. Mol. Руководство по эксплуатации на русском языке, как и на английском, выложена на сайте в Portable Document File формате, очень удобном для скачивания и чтения. Chem. 265:10666-10673. ↵ Heidrich, N., and S. Brantl. 2003. Antisense-RNA mediated transcriptional attenuation: importance of a U-turn loop structure in the target RNA of plasmid pIP501 for efficient inhibition by the antisense RNA. J. Mol.